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KMID : 0545120090190020155
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 2 p.155 ~ p.160
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Kaempferol Isolated from Nelumbo nucifera Stamens Negatively Regulates FcεRI Expression in Human Basophilic KU812F Cells
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Shim Sun-Yup
Choi Jae-Sue
Byun Dae-Seok
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Abstract
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Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, FcεRI. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on FcεRI expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that FcεRI expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RTPCR analysis showed that the mRNA levels for FcεRI α- and γ-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular Ca2+ concentration and histamine release from anti-FcεRI α- chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via downregulation of FcεRI expression and degranulation.
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KEYWORD
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Kaempferol, Nelumbo nucifera stamens, FcεRI, intracellular Ca2+, histamine, KU812F
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